Without nucleic acid extraction, nucleic acid sample can be released at the same time of sampling. 2. QIAamp spin columns can be easily processed in a centrifuge or on vacuum manifolds. Purification of nucleic acids with silica gel membrane products is fast, convenient, and economical. Magnetic-particle technology can often be automated to enable fast and economical nucleic acid purification procedures. Fast, inexpensive 0000003421 00000 n plasmid DNAfor It is convenient for in vitro diagnostic reagents to test the sample and suitable for screening in a large population. Nucleic acid extraction reagent kit for COVID-19. Achieve high yields from challenging tissues that are difficult for many commercial kits (e.g. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Nucleic Acid Extraction Sample Release Kit contains protein deformation and biochemical reagents, which can quickly destroy the protein structure and release nucleic acid, so that the sample release agent can directly amplify nucleic acid after sampling, avoiding the complex nucleic acid extraction process, so that DNA type ultrafast nucleic acid release equipment is simple. You may purchase them with complete confidence!, The Qiagen Dna Extraction Kit Principle came very quickly and no damage, "" , "" , Though with a little late deliver, their service is satisfying., "" , Great Qiagen Dna Extraction Kit Principle for my needs., We can constantly satisfy our respected customers with our good high quality, good price tag and good support due to we have been additional specialist and extra hard-working and do it in cost-effective way for, "Our firm sticks towards the basic principle of ""Quality could be the life of your organization, and name may be the soul of it"" for, Nucleic Acid Extraction Reagent Kit Of COVID-19, One-Step Method Test Solution Of COVID-19, Non-Inactivated Disposable Virus Sampling Tube, Inactivated Disposable Virus Sampling Tube, Nucleic Acid Extraction Reagent Kit 64T(auto), Nucleic Acid Extraction Reagent Kit 96T(auto), Nucleic Acid Extraction Reagent Kit 100T(manual), Nucleic Acid Extraction Reagent Kit 300T(manual), RT-PCR Test Reagent Kit Of COVID-19(ORF1ab, N), RT-PCR Test Reagent Kit Of COVID-19(ORF1ab, N, E), Multiplex RT-PCR test reagent kit Of COVID-19(ORF1ab)/Influenza A/Influenza B, Freeze-Dried RT-PCR Test Reagent Kit Of COVID-19(ORF1ab,N), Freeze-Dried RT-PCR Test Reagent Kit Of COVID-19(ORF1ab, N, E). These products are not intended for the diagnosis, prevention, or treatment of a disease. Structure Search. Name SARS-CoV-2 Antigen Rapid Test Kit, also named Covid-19 Antigen Test Kit, SARS-CoV-2 Antigen Kit, etc. HiSpeed Midi Tips, provided in the HiSpeed Plasmid Midi Kit, contain a newly developed anion-exchange resin. [Product Name] RT-PCR Test COVID-19 Real Time Pcr Test Reagent for testing New coronavirus enables the safe detection of New coronavirus DNA in Saliva samples, Sputum, Nasopharyngeal swabs, Oropharyngeal swabs, Bronchoalveolar lavage fluid(BALF) from hunman beings within 40 minutes. 0000008008 00000 n The MinElute 96 UF PCR Purification Kit provides 96-well plates for high-throughput ultrafiltration-based purification of PCR products 100 bp in size. Available for Sale. Products Building Blocks Explorer Technical Documents Site Content Papers Genes. Name Covid-19 Antigen Test Kit (colloidal gold Successful transfection into sensitive cell lines:Plasmid pCMV DNA was prepared using the indicated preparation method with standard and high-yield (HY) protocols for QIAGEN PlasmidPlusKits or the recommended protocol from the supplier indicated. 0000003643 00000 n Intended Usage The Novel Coronavirus (SARS-CoV-2) Antigen, SARS-CoV-2 Antigen Rapid Test Cassette (Colloidal Gold), Novel Coronavirus Freeze-Dried Multiplex PCR Reagent Kit, Freeze-Dried RT-PCR detection reagent kit, Single-use Viral Sampling Tube (non-inactivated type), Inactivated disposable virus sampling tube, Disposable Medical Collection Viral Transport Medium, New coronavirus nucleic acid testing reagent kit, Freeze-Dried RT-PCR test reagent kit of COVID-19(ORF1ab/N/E), Nucleic acid extraction reagent kit 64T(auto). EZ1&2 DNA Tissue Kit Quick-Start Protocol, Isolation of DNA from soft tissue using the TissueLyser and EZ1 DNA Tissue Kit - (EN), Purification of DNA from fungi using the EZ1 DNA Tissue Kit - (EN), Purification of genomic DNA from whole blood, optimized for use in MRC-Holland MLPA assays, using EZ1 DNA Blood Kits - (EN), (EN) - Comparison of enzymes to lyse streptococcal bacteria priorto automated DNA purification on the BioRobot M48, Automated purification of DNA from bones of a Bronze Age family using the BioRobot EZ1 workstation, (EN) - EZ1 Advanced Automated Solutions Pure Convenience, 'Purification of genomic DNA from whole blood, optimized for use in MRC-Holland MLPA assays, using EZ1 DNA Blood Kits, Isolation of DNA from cultured cells using the EZ1 DNA Tissue Kit, Isolation of genomic DNA from buffy coat using the EZ1 DNA Blood 350 l Kit, Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein, Rapid purification of up to 9.5 g DNA from whole blood, Purification of up to 14 g DNA from buffy coat, Processing of 200350 l whole blood samples, Standardized processing of up to 40 mg tissue samples, Population genetics and pharmacogenomic research, Genotyping analysis, including STR, VNTR, RAPD, NASBA, SNP and AFLP technologies, Insert the appropriateEZ1 ProtocolCard, switch on the workstation, and, if necessary, select the appropriate protocol, Follow the instructions on the liquid-crystal display (LCD), which describes where the sample tubes, elution tubes, filter tips, and EZ1 Reagent Cartridges must be loaded and which indicates when to start the protocol run, Collect purified nucleic acids when the protocol run ends. QIAGEN has developed a wide range of silica gel membrane products that selectively bind either RNA or DNA and separate nucleic acids within certain size parameters. Basic Isolation Procedure. Highly efficient transfection into a sensitive cell line:pCMV DNA was prepared using the indicated preparation method. 0000008295 00000 n QIAGEN silica gel membrane technology yields high-purity nucleic acids suitable for most molecular biology and clinical research applications, such as restriction digestion, ligation, labeling, amplification, and radioactive and fluorescent sequencing. DNeasy kits produce lower yields and low A260/A230 ratios for liver, brain, muscle and frozen blood. optimal results in sensitive A convenient tool to build experimental workflows and find products to match your needs. Purification on QIAGEN resin is based on the interaction between negatively charged phosphates of the nucleic acid backbone and positively charged DEAE groups on the surface of the resin (see figure Binding principle of QIAGEN resin). QIAGEN Plasmid Plus Kits and QIAGEN Plasmid Plus 96 Miniprep and BioRobot Kits provide transfection-grade plasmid DNA, highly suited for all applications such as: QIAGEN Plasmid Plus Kits and QIAGEN Plasmid Plus 96 Miniprep and BioRobot Kits provide transfection-grade plasmid DNA with very low endotoxin levels (see figures Low endotoxin levels, Highly efficient transfection into a sensitive cell line, and Successful transfection into sensitive cell lines). Silica gel membranes are particularly well-suited for use in spin columns or multiwell units designed for high-throughput procedures. Transfer the flow-through fraction from step 5 into a new tube (not supplied) without disturbing the cell-debris pellet. Following genomic DNA extraction is . How much evidence does QIAGEN have. Genomic DNA extraction is the process of releasing chromosomal DNA from the cellular matrix in which it is contained. 0000005829 00000 n Rapid Real time PCR Kit for Monkeypox Virus. Principle Automated purification with EZ1&2 DNA Kits requires use of either EZ2 Connect instruments or EZ1 instruments and EZ1 DNA Cards (see table "EZ1&2 DNA Kits and EZ1 DNA Cards"). Purified nucleic acids are of the highest possible quality and are ideal for sensitive downstream biological applications, such as transfection, microinjection, sequencing, and gene therapy research. Name SARS-CoV-2 Antigen Rapid Test Kit, also named Covid-19 Antigen Test Kit, SARS-CoV-2 Antigen Kit, etc. 0000006129 00000 n As shown in Table 5, QIAGEN QIAamp DNA Blood Maxi Kits produced the highest yield and 260/280 ratio from all methods evaluated, . formatsfor all scales of Our genomic DNA extraction kits overcome these challenges by enabling reproducible genomic DNA isolation from a range of sample types using optimized protocols. Product Name Real time PCR Kit for Monkeypox Virus Product Description This kit is used for in vitro qualitative nucleic acid detection of Monkeypox Virus in Human serum, lesion exudate samples and scab specimens. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 l. 0000005696 00000 n Copyright 2022 Shenzhen Uni-medica Technology Co.,Ltd All rights reserved.Privacy Policy, These are PERFECT! 0000008251 00000 n Achieve high yields from challenging tissues that are difficult for many commercial kits (e.g. startxref After sampling, the virus nucleic acid can be released when mixed with the sample release agent, which saves the cost and time of nucleic acid extraction and can be directly used for subsequent detection. Huh-7 cells (4 x 104) were transfected using 200 ng plasmid DNA and 0.75 l Attractene Transfection Reagent. applications Vaccum, centrifuge, (i) (ii) Explain the principle behind this kit in DNA extraction. This Covid-19 Pcr Diagnostic Reagent kit utilizes a real-time fluorescent PCR detection technology and adopts a pair of specific primers shared by 96-type human papillomavirus (HPV) and a specific fluorescent probe to realize qualitative detection of 96-type HPV pathogens and COVID-19. Compare Product No. Greener Category. HWmH_QOX74DQynv9F6^a0_U7` fv/MSUO=U~>>~\?~ >}{T*-$>>fl`-V8N%>}6 and automated 0000018549 00000 n 0000006515 00000 n The operation is convenient and simple, and non-professionals can also master it quickly Automatic Nucleic acid extraction reagent kit. Product Name Multiplex Real Time PCR Kit for SARS-CoV-2/Influenza A/Influenza B and RSV Alternative Name Multiplex Real Time PCR Kit for Covid-19+Influenza A/Influenza B+RSV, also named Real Time multiplex PCR test reagent kit for Covid-19+Influenza A/ B+RSV, etc. Here we applied QIAGEN Clotspin Baskets and the Gentra Puregene Blood Kit for DNA extraction from 5.5 ml whole blood without anticoagulating additives. Separation of nucleic acids at neutral pH on anion-exchange resins. How do I safely inactivate biohazardous flow-through material? Typically 450 L of lysate is recovered. Delivers ultrapure, 0000001200 00000 n You can obtain high yields of high-quality DNA, even from specialized samples. This Rt Pcr Test Reagent kit consists of fluorescence PCR mix Automatic high throughput nucleic acid extraction instrument. Optimized high-yield protocols and extra buffer volumes are provided with the kit, enabling yields from 250 g (Midi) to 10 mg (Giga). nucleic acids for DNA ranging from 70 bp to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30-50 l. Shenzhen Uni-medica Technology Co.,Ltd . Silica-membrane technology yields high-purity nucleic acids suitable for most molecular biology and clinical research applications, such as restriction digestion, ligation, labeling, amplification, and radioactive and fluorescent sequencing. Refrigerated samples and reagents from the kit are brought to room temperature before starting the procedure. The genomic purification kits are available in both bead-based and spin-column formats to suit your needs. Yes, please follow the Supplementary Protocol. The DNA release reagent is suitable for the preserve, Magnetic particle technology combines the speed and efficiency of silica-based DNA purification with convenient handling and ease of automation. These DNA extraction kits make the whole process much easier and faster than the DNA isolation methods of old when things are going well. in vitro detection. Purification using QIAGEN silica gel membrane technology is based on a simple bind-wash-elute procedure. DNA can be purified using many different methodsand the downstream application determines how pure the DNA should be. 0000001844 00000 n The EZ1 DNA Blood 200 l Kit and EZ1 DNA Blood 350 l Kit are for purification of genomic DNA from whole blood samples and blood products. 56404) provide special Apparatus: 1.5mL eppendorf/microcentrifuge tubes, centrifuge, block heater, incubator, timer, ice, micropipette, Qiagen DNA extraction kit. no alcohol precipitation, Delivers high-purity View Got a technical question? purification of nucleic acid. After a wash step, pure nucleic acids are eluted under low- or no-salt conditions in small volumes, ready for immediate use without further concentration. The DNA of interest is bound to a special membrane, the remaining sample is washed out and then the DNA of interest is eluted. Product Name COVID-19 Rapid Antigen Detection Kit Alternative EZ1&2 DNA Kits are intended for molecular biology applications. Mr. Jack . The DNA kit recommends QIAamp viral RNA Mini Kit for preparation of cellular, bacterial, or viral DNA from urine, because the buffer AVL in RNA kit is optimized to inactivate the PCR inhibitors present in urine. The resin has a higher capacity, allowing higher yields of high-copy plasmid DNA to be obtained from HiSpeed Midi Tips than from classic midi tips. processing options, Delivers high-purity DNeasy-purified gDNA peak sizes are typically <30 kb with DINs ~7-8. <<063195965738634F93F311AD02245056>]>> What makes QIAGEN so popular for RNA extraction and DNA isolation? The DNA is ready to use, or can be stored at 28C for 24 h, or at 20C for longer periods. DNeasy Blood & Tissue Kits are designed for rapid purification of total DNA (e.g., genomic, mitochondrial, and pathogen) from a variety of sample sources including fresh or frozen animal tissues and cells, blood, or bacteria. %%EOF QIAGEN offers a wide range of specialized nucleic acid purification products based on four highly developed purification technologies: solid-phase, anion-exchange technology; QIAGEN Plasmid Plus technology; silica gel membrane technology; and magnetic particle technology. For example, add 5 l of 10x Forensic TE buffer if you eluted in 50 l. The principle of this technology is simple. The procedure can be performed in 20 (Midi and Maxi), 40 (Mega), or 50 minutes (Giga) using a vacuum and centrifuge. Do you have a protocol for the isolation of DNA from buffy coat using the BioRobot EZ1? DNeasy kits produce lower yields and low A260/A230 ratios for liver, brain, muscle and frozen blood. transfection-grade Incubate at 50C for 10 minutes (or until the gel slice has completely dissolved). All Photos (4) Agarose Gel DNA Extraction Kit. The characteristics of the 3 most common types of DNA extraction kit are shown in the table. DNeasy-purified gDNA peak sizes are typically <30 kb with DINs ~7-8. Working with DNA: Good laboratory practice, Sample disruption for extraction of genomic DNA, Special considerations for isolating genomic DNA from different sample sources, Restriction endonuclease digestion of DNA, Selecting suitable restriction endonucleases, Sample storage prior to extraction of genomic DNA T, Animal, yeast, and bacterial cell cultures, Spectrophotometric measurement of DNA concentration, Preparation of gels for Southern blotting, Solid-phase, anion-exchange chromatography, Binding to magnetic silica particles under, Delivers ultrapure, transfection-grade DNA, Delivers high-purity nucleic acids for use in most, Delivers high-purity nucleic acids for use in, No silica-slurry carry over, no alcohol precipitation, Easy to automate; no alcohol precipitation. For high-throughput, small-scale purification of BACs, PACs, and P1s, an optimized alkaline lysis protocol in R.E.A.L. trailer QIAGEN technologies have revolutionized nucleic acid purification by substantially reducing preparation times and eliminating the need for costly equipment, such as ultracentrifuges, and toxic chemicals, such as phenol. If you can't find the product you're looking for, please contact us for more information. xref EZ1 Cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure. How to store DNA from forensic samples eluted into water on the EZ1 biorobot in TE buffer instead? zqa}dS.xZXn~_. Advanced Search. Prep 96 Kits yields DNA suitable for sequencing and screening. Small-to large-scale plasmid purification, High-molecular-weight genomic DNA isolation, QIAGEN Plasmid Plus 96 Miniprep and BioRobot Kits, DNA isolation from animal tissues and cells, DNA and RNA isolation from various samples, Transfection into most cell lines (including sensitive cell lines such as Huh-7), Preparation of short hairpin vectors (sh-vectors). The quality of silica gel membranes used in QIAGEN products ensures consistent yields of high-purity nucleic acids. Do you have a protocol for the isolation of DNA from buccal cells using the BioRobot EZ1? Sales at CER were $533 million, ahead of the outlook for at least $510 . Reagent Alternative Name DNA Extraction Reagent, 0000003193 00000 n The process does not require the extraction of nucleic acid, which greatly reduces the cost of operation. 0000000832 00000 n DNA extraction kits have also evolved, . method), also named 2019-nCoV antigen Recombinant DNA Principles and Methodologies. Cl, 1 mM EDTA, pH 8.3) to each sample. Plasmid DNA prepared with QIAGEN Plasmid Plus 96 Miniprep and BioRobot Kits resulted in highly efficient transfection into sensitive cell lines. Mr. Jack . endstream endobj 322 0 obj<>/Outlines 19 0 R/Metadata 165 0 R/PieceInfo<>>>/Pages 164 0 R/PageLayout/OneColumn/OCProperties<>/StructTreeRoot 167 0 R/Type/Catalog/LastModified(D:20090309090955)/PageLabels 162 0 R>> endobj 323 0 obj<>/PageElement<>>>/Name(HeaderFooter)/Type/OCG>> endobj 324 0 obj<>/Font<>/ProcSet[/PDF/Text]/Properties<>/ExtGState<>>>/Type/Page>> endobj 325 0 obj<> endobj 326 0 obj<> endobj 327 0 obj[/ICCBased 341 0 R] endobj 328 0 obj<> endobj 329 0 obj<> endobj 330 0 obj<> endobj 331 0 obj<>stream Genomic DNA extraction requires a robust disruption method to open the nuclei and cell walls (if applicable); it usually involves adding a compatible detergent as well as mechanical shearing. To help dissolve the gel, mix by vortexing the tube every 2-3 minutes during the incubation period. This Nucleic acid extraction reagent is package of 64 tests/kit, we can also offer custom package. Comparison of QIAGEN nucleic acid purification technologies. Product Name Nucleic Acid Extraction carry over, Protocols can be carried out manually (using either a microcentrifuge or a vacuum manifold*) or fully automated on the QIAcube. of purification 0000002170 00000 n VENLO, Netherlands--(BUSINESS WIRE)-- QIAGEN N.V. (NYSE: QGEN; Frankfurt Prime Standard: QIA) announced results for the third quarter of 2022 and first nine months of 2022, and increased the outlook for full-year 2022. of the Multiplex Real Time PCR Kit for SARS-CoV-2/Influenza A/Influenza B and RSV. And then without extraction kit, professional equipment and professional testing cost and time DNA type ultrafast nucleic acid release reagent kit. DNA extraction kits are . Fast, inexpensive Without nucleic acid extraction, the sample can be directly after sampling for amplification reaction, avoid cross infection in the process of extracting samples.
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